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Suppressive Effect of Hochu-Ekki-To on Collagen Induced Arthritis in DBA1J Mice
LE XUAN HAI, TOSHIAKI KOGURE, ATSUSHI NIIZAWA, HIROSHI FUJINAGA, IWAO SAKAKIBARA, YUTAKA SHIMADA, HIROSHI WATANABE, and KATSUTOSHI TERASAWA
ABSTRACT.
Methods. CIA was induced in male DBA/1J mice by immunization with 2 injections of bovine type II collagen (CII). HET was orally administered at different doses and with different schedules. The incidence of arthritis, arthritis index, levels of anti-CII antibody, interleukin 6 (IL-6), and tumor necrosis factor-a (TNF-a) and lymphocyte subsets were examined. Results. HET caused suppression of CIA development in a dose dependent fashion and exerted a suppressive effect on CIA when administered from the first CII immunization or from the onset of CIA, but not when administered for 2 weeks before CII immunization. HET inhibited the production of specific anti-CII antibody, IL-6, and TNF-a, and tended to normalize the proportions of cells in lymphocyte subsets. Conclusion. HET suppresses the development of CIA, and HET redistributes the population of lymphocytes in lymph node and blood and inhibits IL-6 and TNF-a secretion in CIA mice. (J Rheumatol 2002;29:1601-8) Key Indexing Terms:
HOCHU-EKKI-TO (BU-ZHONG-YI-QI-TANG)
From the Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University; Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University; Kampo & Pharmacognosy Laboratory, Tsumura & Co; and Department of Japanese Oriental Medicine, Toyama Prefectural Central Hospital, Toyama, Japan. L.X. Hai, MD; T. Kogure, MD, PhD, Lecturer Professor; A. Niizawa, MD, Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University; H. Fujinaga, MD, Department of Japanese Oriental Medicine, Toyama Prefectural Central Hospital; I. Sakakibara, PhD, Kampo & Pharmacognosy Laboratory, Tsumura & Co.; Y. Shimada, MD, PhD, Associate Professor, Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University; H. Watanabe, PhD, Professor Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University; K. Terasawa, MD, PhD, Professor, Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University. Address reprint requests to Dr. T. Kogure, Department of Japanese Oriental Medicine, School of Medicine, Gunma University, Showa-machi 3-39-22, Maebashi Gunma 371-8511, Japan. E-mail: tkogure@showa.gunma-u.ac.jp Submitted May 30, 2001; revision accepted February 27, 2002.
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