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Differential Expression Patterns of Secreted Frizzled Related Protein Genes in Synovial Cells from Patients with Arthritis KOSEI IJIRI, RYUSAKU NAGAYOSHI, NORIKO MATSUSHITA, HIROMICHI TSURUGA, NOBORU TANIGUCHI, AKIRA GUSHI, HARUTOSHI SAKAKIMA, SETSURO KOMIYA, and TAKAMI MATSUYAMA
ABSTRACT. Methods. Expression of sFRP-1, 2, 3, 4, and 5 genes was detected in synovial cells from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. To identify synovial cell populations expressing sFRP-1, 3, and 4 genes, expression was compared in macrophage-rich populations and fibroblast-like cell-rich populations by RT-PCR. Levels of expression of these genes were also studied in activated peripheral blood mononuclear cells (PBMC) and activated skin fibroblasts. Results. Expression of the sFRP-1, 3, and 4 genes was observed in both RA and OA synovial cells. sFRP-1 and 4 genes were expressed predominantly in fibroblast-like cell-rich populations, and the sFRP-3 gene was expressed predominantly in macrophage-rich populations. Levels of expression of sFRP-3 and 4 genes were elevated in activated PBMC and activated skin fibroblasts. Conclusion. Our findings suggest that sFRP-1, 3, and 4 may play different roles in the pathogenesis of synovitis. (J Rheumatol 2002;29:2266-70) Key Indexing Terms:
SYNOVIAL CELLS
From the Department of Orthopaedic Surgery, Faculty of Medicine; Course of Physical Therapy, Faculty of Medicine; and Department of Immunology and Medical Zoology, Kagoshima University, Kagoshima, Japan.
Supported by a grant to K. Ijiri (00315417) from the Ministry of Culture, Education and Science of Japan.
K. Ijiri MD, PhD, Department of Orthopaedic Surgery, Course of Physical Therapy, Faculty of Medicine; R. Nagayoshi, MD, Department of Orthopaedic Surgery; N. Matsushita, BSc; H. Tsuruga, PhD, Department of Immunology and Medical Zoology; N. Taniguchi, MD, Department of Orthopaedic Surgery; A. Gushi, MD, Department of Immunology and Medical Zoology; H. Sakakima, MSc, Course of Physical Therapy, Faculty of Medicine; S. Komiya, MD, PhD, Department of Orthopaedic Surgery; T. Matsuyama, MD, PhD, Department of Immunology and Medical Zoology.
Address reprint requests to Dr. S. Komiya, Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University, 8-35-1, Sakuragaoka, 890-8520 Kagoshima, Japan.
Submitted December 27, 2001; revision accepted May 6, 2002.
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