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Expression of Cyclin B1 and Cyclin Dependent Kinase Inhibitor p21 in Lymphocytes in Patients with Systemic Lupus Erythematosus
CHEONG-YIP HO, CHUN-KWOK WONG, EDMUND KWOK-MING LI, LAI-SHAN TAM, and CHRISTOPHER WAI-KEI LAM
ABSTRACT.
Methods. PBL from 40 SLE patients with renal disease (RSLE), 40 SLE patients without renal disease (SLE), and 28 healthy control subjects were cultured with phytohemagglutinin (PHA). Bivariate distribution of cyclin B1 or p21 expression versus cellular DNA content was assessed by flow cytometry. Results. Expression of p21 in lymphocytes was significantly lower in patients with RSLE and with SLE than controls (RSLE vs controls and SLE vs controls, both p < 0.001). Expression of cyclin B1 was similar in all groups. The percentages of RSLE lymphocytes in G0/G1 and S phase were significantly reduced and elevated, respectively, compared with controls. Conclusion. Downregulated p21 in PHA stimulated PBL from patients with SLE may be closely related to aberration of cell division in SLE lymphocytes. (J Rheumatol 2002;29:2537-44) Key Indexing Terms:
SYSTEMIC LUPUS ERYTHEMATOSUS
From the Department of Chemical Pathology and Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong. Supported by a Direct Grant for Research of The Chinese University of Hong Kong and a donation from Zindart (De Zhen) Foundation Ltd., Hong Kong. C.Y. Ho, PhD, Postdoctoral Fellow; C.K. Wong, PhD, Associate Professor, Department of Chemical Pathology; E.K. Li, MD, Associate Professor; L.S. Tam, MD, Medical Officer, Department of Medicine and Therapeutics; C.W.K. Lam, PhD, Professor, Department of Chemical Pathology. Address reprint requests to Prof. C.W.K. Lam, Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong. E-mail: waikeilam@cuhk.edu.hk Submitted January 14, 2002; revision accepted June 11, 2002.
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