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Lack of Association of Ankylosing Spondylitis
with the Most Common NOD2 Susceptibility
Alleles to Crohn's Disease
ISABEL FERREIRÓS-VIDAL, JUAN AMARELO, FRANCISCO BARROS, ANGEL CARRACEDO, JUAN J. GÓMEZ-REINO, and ANTONIO GONZALEZ
ABSTRACT.
Methods. DNA from 112 patients with AS and 168 controls of homogenous Spanish ancestry were studied. The frequencies of the pathogenic alleles of NOD2 (3020insC, 2722G>C, and 2104C>T) were determined by analysis of the melting curves after hybridization with FRET probes on a Light Cycler real-time polymerase chain reaction (PCR) system. Results. NOD2 allelic frequencies in controls (3020insC, 0.009; 2722G>C, 0.009; 2104C>T, 0.042) did not significantly differ from patients with AS (3020insC, 0.009; 2722G>C, 0.004; 2104C>T, 0.031). Conclusion. The 3 most common CD NOD2 mutations do not contribute to disease susceptibility to AS, and therefore do not explain the susceptibility locus for AS in chromosome 16q. (J Rheumatol 2003;30:102-4) Key Indexing Terms:
ANKYLOSING SPONDYLITIS
From the Research Laboratory 2 and Rheumatology Unit, Hospital Clinico Universitario de Santiago, and the Molecular Medicine Unit-INGO, Universidad de Santiago de Compostela, Santiago de Compostela, Spain. Supported by grant 01/3138 of the Fondo de Investigaciones Santiarias, MSC (Spain) and Schering-Plough Spain. I. Ferreirós-Vidal, MA; J. Amarelo, MD; J.J. Gómez-Reino, MD, PhD; A. Gonzalez, MD, PhD, Research Laboratory 2 and the Rheumatology Unit, Hospital Clinico Universitario de Santiago; F. Barros, PhD; A. Carracedo, MD, PhD, Molecular Medicine Unit-INGO, Universidad de Santiago de Compostela. Address reprint requests to Dr. A. Gonzalez, Laboratorio de Investigación 2, Hospital Clinico Universitario de Santiago, 15706 Santiago de Compostela, Spain. Submitted January 17, 2002; revision accepted June 27, 2002. |