Intermittent Hydrostatic Pressure Inhibits Shear Stress-Induced Nitric Oxide Release in Human Osteoarthritic Chondrocytes in Vitro
MEL S. LEE, MICHAEL C.D. TRINDADE, TAKASHI IKENOUE, DAVID J. SCHURMAN, STUART B. GOODMAN, and R. LANE SMITH
Methods. Chondrocytes isolated from cartilage samples from 9 patients with osteoarthritis were cultured and exposed to either shear stress or an NO donor. Nitrite concentration was measured using the Griess reaction. Matrix macromolecule mRNA signal levels were determined using reverse-transcriptase polymerase chain reaction and quantified by imaging analysis software.
Results. Exposure to shear stress upregulated NO release in a dose and time-dependent manner. Application of IHP inhibited shear stress induced NO release but did not alter NO release from chondrocytes not exposed to shear stress. Shear stress induced NO or addition of an NO donor (sodium nitroprusside) was associated with decreased mRNA signal levels for the cartilage matrix proteins, aggrecan, and type II collagen. Intermittent hydrostatic pressure blocked the inhibitory effects of sodium nitroprusside but did not alter the inhibitory effects of shear stress on cartilage macromolecule gene expression.
Conclusion. Our data show that shear stress and IHP differentially alter chondrocyte metabolism and suggest that a balance of effects between different loading forces preserve cartilage extracellular matrix in vivo. (J Rheumatol 2003;30:326-8)
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From the Orthopaedic Research Laboratory and the Division of Orthopaedic Surgery, Stanford University School of Medicine, Stanford; and the RR&D Center, Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.
Supported by NIH Grant AR45788.
M.S. Lee, MD, Assistant Professor, Chang Gung Memorial Hospital, Taiwan, ROC; M.C.D. Trindade, MS, Medical Student; T. Ikenoue, MD, Postdoctoral fellow; R.L. Smith, PhD, Professor (Research), Orthopaedic Research Laboratory; D.J. Schurman, MD, Professor; S.B. Goodman, MD, PhD, Professor, Division of Orthopaedic Surgery.
Address reprint requests to Dr. R.L. Smith, Orthopaedic Research Laboratory, Stanford University School of Medicine, 300 Pasteur Drive, R144, Stanford, CA, 94305-5341, USA. E-mail: email@example.com
Submitted March 14, 2002; revision accepted June 4, 2002.