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Lack of Association Between Endothelial Nitric
Oxide Synthase Polymorphisms and Henoch-Schönlein Purpura
MAHSA M. AMOLI, CARLOS GARCIA-PORRUA, MARIA C. CALVIÑO, WILLIAM E.R. OLLIER, and MIGUEL A. GONZALEZ-GAY
ABSTRACT.
Methods. Fifty patients from Northwest Spain with primary cutaneous vasculitis classified as HSP were studied. Patients and ethnically matched controls (n = 117) were genotyped by polymerase chain reaction techniques for a variable-number tandem-repeat polymorphism in intron 4, a T/C polymorphism at position –786 in the promoter region, and a polymorphism in exon 7 (298Glu/Asp or 5557G/T) of the eNOS gene. Results. No differences in allele or genotype frequencies for any of the individual eNOS polymorphisms were observed between patients fulfilling HSP classification criteria and controls, or when patients were stratified for the presence of nephritis or joint or gastrointestinal manifestations. In the HSP group no linkage disequilibrium between these polymorphisms was found. No significant difference in haplotype frequencies was observed between patients and controls. Conclusion. Our results do not support a role for these polymorphisms in the susceptibility to HSP. (J Rheumatol 2004;31:299-301) Key Indexing Terms:
HENOCH-SCHÖNLEIN PURPURA
From the Centre for Integrated Genomic Medical Research, School of Epidemiology and Health Sciences, the University of Manchester, Manchester, United Kingdom; and the Divisions of Rheumatology and Pediatrics, Hospital Xeral-Calde, Lugo, Spain. M.M. Amoli, MD, PhD; W.E.R. Ollier, PhD, FRCPath, Centre for Integrated Genomic Medical Research, University of Manchester; M.A. Gonzalez-Gay, MD, PhD; C. Garcia-Porrua, MD, PhD, Rheumatology Division; M.C. Calviño, MD, Pediatrics Division, Hospital Xeral-Calde. Address reprint requests to Dr. M.A. Gonzalez-Gay, Rheumatology Division, Hospital Xeral-Calde, Dr. Ochoa s/n, 27004 Lugo, Spain. E-mail: miguelaggay@hotmail.com Submitted March 11, 2003; revision accepted July 4, 2003. |