Cytochrome P450 1A1 and Manganese Superoxide Dismutase Gene Polymorphisms in Behçet's Disease

JENG-HSIEN YEN, WEN-CHUN TSAI, CHIA-HUI LIN, TSAN-TENG OU, CHAUR-JONG HU, and HONG-WEN LIU

ABSTRACT.

Objective. To investigate the association of cytochrome p450 1A1 (CYP1A1) and manganese superoxide dismutase (MnSOD) gene polymorphisms with susceptibility to Behçet's disease (BD) in Taiwan.

Methods. The polymorphisms of CYP1A1 and MnSOD genes were determined in 51 patients with BD and 91 healthy controls by polymerase chain reaction/restriction fragment length polymorphism methods.

Results. The frequencies of CYP1A1 4889G and 4887A were significantly increased in patients with BD. In contrast, there was no significant difference in the frequencies of MnSOD gene polymorphisms between patients with BD and controls. Linkage disequilibrium was found between CYP1A1 4889G and CYP1A1 6235C in controls and patients with BD. However, a similar finding could not be found between CYP1A1 4889G and 4887A. We also found that the association of CYP1A1 4889G with BD was dependent on the presence of CYP1A1 4887A. On the other hand, the association of CYP1A1 4887A with BD was dependent on the presence of CYP1A1 4889G. An additive effect of CYP1A1 4889G and 4887A on the susceptibility to BD could be found.

Conclusion. Simultaneous presence of CYP1A1 4889G and 4887A is associated with development of BD in Taiwan. (J Rheumatol 2004;31:736-40)

Key Indexing Terms:

MANGANESE SUPEROXIDE DISMUTASE
CYTOCHROME
BEHÇET'S DISEASE

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From the Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital; Department of Medicine, Kaohsiung Municipal United Hospital; Department of Internal Medicine, Kaohsiung Municipal Hsiao-Kang Hospital; and the Molecular Medicine Laboratory, Taipei Municipal Jen-Ai Hospital, Taiwan.

J-H. Yen, MD, PhD, MSc, Professor, Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Department of Medicine, Kaohsiung Municipal United Hospital; W-C. Tsai, MD, PhD, Associate Professor; C-H. Lin, Technician; T-T. Ou, MD, Attending Physician; H-W. Liu, MD, Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Professor, Kaohsiung Medical University; C-J. Hu, MD, Lecturer, Molecular Medicine Laboratory, Taipei Municipal Jen-Ai Hospital.

Address reprint requests to Dr. J-H. Yen, No. 100 Shih-Chuan 1st Road, Kaohsiung City 807, Taiwan. E-mail: jehsye@kmu.edu.tw

Submitted January 20, 2003; revision accepted September 26, 2003.

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