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Abnormal Distribution of Aquaporin-5 in Salivary Glands in the NOD Mouse Model for Sjögren's Syndrome
YRJÖ T. KONTTINEN, EVE-KAI TENSING, MIKAEL LAINE, PAULIINA POROLA, JYRKI TÖRNWALL, and MIKA HUKKANEN
ABSTRACT.
Objective. To localize aquaporin-5 in healthy salivary gland acinar cells and to check if it is abnormally translocated in an experimental NOD mouse model for Sjögren's syndrome (SS). Methods. Healthy BALB/c control mice and autoimmune focal adenitis NOD mice were studied. Aquaporin-5 was stained using avidin-biotin-peroxidase complex and indirect immunofluorescence staining methods, and visualized using light and laser scanning confocal microscopy. Results. Aquaporin-5 was found in the apical domain of the acinar cell plasma membrane in healthy BALB/c mice. In contrast, aquaporin-5 was found in the apical and basolateral acinar plasma cell membrane in parotid, submandibular, and sublingual glands in NOD mice. This was confirmed using laser scanning confocal microscopy for optical sectioning and image reconstruction. Conclusion. Our findings reveal an abnormal translocation of aquaporin-5 in an experimental SS animal model and support observations that implied a similar loss of the ordered and polarized expression of aquaporin-5 in human SS labial and lacrimal glands. (J Rheumatol 2005;32:1071-5) Key Indexing Terms:
AQUAPORIN-5
From the Department of Medicine/Invärtes medicin, Helsinki University Central Hospital, ORTON Orthopaedic Hospital of the Invalid Foundation, Helsinki; COXA Hospital for Joint Replacement, Tampere; and Department of Anatomy, Institute of Biomedicine, University of Helsinki, Helsinki, Finland. Supported by Finska Läkaresällskapet, HUS evo grant, Invalid Foundation, the Finnish Dental Society Apollonia, the Centre for International Mobility, and the University of Helsinki. Y.T. Konttinen, MD, PhD, Department of Medicine/Invärtes medicin, Helsinki University Central Hospital, ORTON Orthopaedic Hospital of the Invalid Foundation, Helsinki, COXA Hospital for Joint Replacement, Tampere; E-K. Tensing, MD, Department of Medicine/Invärtes medicin, Helsinki University Central Hospital; M. Laine, DDS, Medical Student, Department of Anatomy, Institute of Biomedicine, University of Helsinki; P. Porola, BSc, Department of Anatomy, Institute of Biomedicine, University of Helsinki; J. Törnwall, MD, Department of Anatomy, Institute of Biomedicine, University of Helsinki; M. Hukkanen, PhD, Department of Anatomy, Institute of Biomedicine, University of Helsinki. Address reprint requests to Prof. Y.T. Konttinen, Department of Medicine/invärtes medicin, Biomedicum, PO Box 700, Haartmaninkatu 8, FIN-00029 HUS, Finland. E-mail: yrjo.konttinen@helsinki.fi Accepted for publication January 24, 2005. |