Association of Nuclear Factor-kB in Psoriatic Arthritis

CHRISTOPHER BUTT, SHUYING SUN, LYNETTE PEDDLE, CELIA GREENWOOD, SEAN HAMILTON, DAFNA GLADMAN, and PROTON RAHMAN

ABSTRACT.

Objective. To examine the association of single nucleotide polymorphisms (SNP) in the NFKB1 gene, as well as 2 genes in the nuclear factor (NF)-kB functional complex (RelA and NFKBIA), in patients with psoriatic arthritis (PsA) from Newfoundland.

Methods. Patients with PsA and controls were genotyped for one 4-base insertion/deletion and 5 SNP in NFKB1, 4 SNP in RelA, and 7 SNP in NFKBIA by time-of-flight mass spectrometry, using the Sequenom platform. Chi-square analysis was used to test the single locus associations between SNP in the NF-kB complex and PsA. Associations between multi-locus haplotypes and case or control status were tested using the software PHASE.

Results. Two hundred and twenty-four patients with PsA (52% male) and 88 ethnically matched controls (64% male) were genotyped. No association was noted with any of the SNP tested for the single locus associations in NFKB1, RelA, and NFKBIA or with multi-locus haplotypes. In particular, the allele frequency for the NFKB1 -94delATTG was 41.7% in cases and 41.6% in the controls (p = 0.97).

Conclusion. No association between the NFKB1 -94 ins/delATTG promoter polymorphism or with other NF-kB complex SNP in patients with PsA from Newfoundland was observed. (J Rheumatol 2005;32:1742-4)

Key Indexing Terms:

PSORIATIC ARTHRITIS
SINGLE NUCLEOTIDE POLYMORPHISM
NUCLEAR FACTOR KAPPA B
PATHOGENESIS

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From Memorial University of Newfoundland (MUN), Genetics and Genomic Biology, Hospital for Sick Children, Department of Public Health Sciences, University of Toronto, and Toronto Western Hospital, Toronto, Ontario, Canada.

C. Butt, MSc, Graduate Student, MUN; S. Sun, Graduate Student, Department of Statistics, University of Toronto; L. Peddle, BSc, Laboratory Scientist, MUN; C. Greenwood, Project Director, Program in Genetics and Genomic Biology, Hospital for Sick Children; S. Hamilton, MD, Associate Professor of Medicine, Division of Rheumatology, MUN; D.D. Gladman, MD, FRCPC, Professor of Medicine, University of Toronto and Director, Psoriatic Arthritis Program, Senior Scientist, Toronto Western Research Institute; P. Rahman, MD, MSc, FRCPC, Associate Professor of Medicine, Division of Rheumatology, MUN.

Address reprint requests to Dr. P. Rahman, St Clare's Mercy Hospital, 1 South - 154 LeMarchant Rd., St. John's, Newfoundland, A1C 5B8. E-mail:prahman@mun.ca

Accepted for publication May 2, 2005.

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