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Lack of Association Between Macrophage Migration Inhibitory Factor Gene Polymorphism and
Giant Cell Arteritis
MAHSA M. AMOLI, CARLOS GARCIA-PORRUA, WILLIAM E.R. OLLIER, and MIGUEL A. GONZALEZ-GAY
ABSTRACT. Methods. Eighty-three patients with biopsy-proven GCA, 20 of them with visual ischemic complications, and 122 healthy matched controls from the Lugo region of Northwest Spain were studied. Patients and controls were genotyped for a single nucleotide polymorphism in the 5'-flanking region at position –173 of the MIF gene, using SNapshot ddNTP primer extension, followed by capillary electrophoresis (ABI 3100). Results. No significant differences in MIF gene polymorphism were observed in patients with biopsy-proven GCA compared to controls. This was also the case when GCA patients with or without visual ischemic complications were compared. Conclusion. Polymorphism in MIF gene promoter –173 G/C does not appear to be a genetic risk factor for GCA in Northwest Spain. (J Rheumatol 2005;32:74-6) Key Indexing Terms:
GIANT CELL ARTERITIS
From the Centre for Integrated Genomic Medical Research, School of Epidemiology and Health Sciences, the University of Manchester, Manchester, UK, and the Division of Rheumatology, Hospital Xeral-Calde, Lugo, Spain. M.M. Amoli, MD, PhD; W.E.R. Ollier, PhD, FRCPath, Centre for Integrated Genomic Medical Research, School of Epidemiology and Health Sciences, University of Manchester; C. Garcia-Porrua, MD, PhD; M.A. Gonzalez-Gay, MD, PhD, Division of Rheumatology, Hospital Xeral-Calde. Address reprint requests to Dr. M.A. Gonzalez-Gay, Rheumatology Division, Hospital Xeral-Calde, c) Dr. Ochoa s/n, 27004 Lugo, Spain. E-mail: miguelaggay@hotmail.com Submitted November 14, 2003; revision accepted July 28, 2004. |