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Glutathione S-Transferase M1, T1, and P1 Genotypes and Rheumatoid Arthritis
BO RA YUN, AHMED EL-SOHEMY, MARILYN C. CORNELIS, and SANG-CHEOL BAE
ABSTRACT.
Objective. To determine the effects of genetic polymorphisms of glutathione S-transferase (GST) M1, GSTT1, and GSTP1 on risk and severity of rheumatoid arthritis (RA) in a Korean population. Methods. A total of 258 patients with RA and 400 disease-free controls were enrolled. GST genotypes were determined by RFLP-PCR. HLA-DRB1 typing and further subtyping of all alleles was performed using sequence-specific oligonucleotide probe hybridization after PCR. Severity of RA among cases was assessed by Steinbrocker anatomical stage. Risk was assessed by calculating the age and sex adjusted odds ratio (OR) and 95% confidence intervals (CI). Results. The OR for risk of RA with the GSTM1-null genotype was 1.40 (95% CI 1.02– 1.92, p = 0.04), and 1.86 (95% CI 1.12– 3.09, p = 0.005) among individuals without the shared epitope (SE). Among patients with RA, the OR for risk of severe RA for the GSTM1-null genotype was 2.45 (95% CI 1.04– 5.77, p = 0.02). No association was observed between the GSTT1 or GSTP1 genotypes and either risk or severity of RA. Conclusion. These results suggest that the deletion polymorphism of GSTM1 is associated with increased susceptibility for RA, particularly among individuals who are not carriers of the HLA-DRB1 SE. (J Rheumatol 2005;32:992-7) Key Indexing Terms:
RHEUMATOID ARTHRITIS
From the Department of Internal Medicine, Division of Rheumatology, and the Hospital for Rheumatic Diseases, Hanyang University, Seoul, Korea; and Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada. Supported in part by a grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (01-PJ3-PG6-01GN11-0002). B.R. Yun, MD, PhD; S.C. Bae, MD, PhD, MPH, Hospital for Rheumatic Diseases, Hanyang University; A. El-Sohemy, PhD; M.C. Cornelis, BSc, Department of Nutritional Sciences, University of Toronto. Address reprint requests to Dr. S-C. Bae, The Hospital for Rheumatic Diseases, Hanyang University Medical Center, Seoul 133-792, Korea. E-mail: scbae@hanyang.ac.kr Accepted for publication January 10, 2005. |