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SLC22A4, RUNX1, and SUMO4 Polymorphisms Are Not Associated with Rheumatoid Arthritis:
A Case-Control Study in a Spanish Population
GISELA OROZCO, ELENA SÁNCHEZ, MIGUEL A. GONZÁLEZ-GAY, MIGUEL A. LÓPEZ-NEVOT, BELÉN TORRES, DORA PASCUAL-SALCEDO, ALEJANDRO BALSA, JOSE L. PABLOS, ANTONIO GARCÍA, Ma FRANCISCA GONZÁLEZ-ESCRIBANO, and JAVIER MARTÍN ABSTRACT.
Objective. To replicate the association reported in Japanese individuals of functional SLC22A4 and RUNX1 polymorphisms with rheumatoid arthritis (RA), and to test the possible role in this trait of a functional variant of the SUMO4 gene that was shown to be associated with another related autoimmune disease, type 1 diabetes (T1D).
Methods. Our study population consisted of 886 patients with RA and 987 healthy controls. All subjects were of Spanish Caucasian origin. We conducted a case-control association study with 6 single-nucleotide polymorphisms (SNP) spanning the SLC22A4 gene. SNP mapping in the RUNX1 gene associated with RA in a Japanese population and a SUMO4 polymorphism associated with T1D were also studied. Results. No statistically significant differences between patients with RA and healthy controls were observed when comparing the distribution of the genotypes or alleles of any of the SLC22A4 polymorphisms tested. Similarly, no evidence of association between RA and the SLC22A4 haplotype previously reported to be associated in a Japanese population was found. With regard to the RUNX1 and SUMO4 SNP, we did not observe statistically significant differences in the distribution of genotypes or alleles between patients with RA and healthy controls. Conclusion. These results suggest that the SLC22A4, RUNX1, and SUMO4 polymorphisms analyzed do not confer a relevant role in susceptibility to RA in the Spanish population. (J Rheumatol 2006;33:1235–9) Key Indexing Terms:
RHEUMATOID ARTHRITIS
From the Instituto de Parasitología y Biomedicina, Granada; Servicio de Reumatología, Hospital Xeral-Calde, Lugo; Servicio de Inmunología, Hospital Virgen de las Nieves, Granada; Servicio de Inmunología, Hospital Virgen del Rocío, Sevilla; Servicio de Reumatologia e Inmunología, Hospital La Paz, Madrid; Servicio de Reumatología, Hospital 12 de Octubre, Madrid; and Servicio de Reumatología, Hospital Virgen de las Nieves, Granada, Spain. Supported by Plan Nacional de I+D (grant SAF03-3460), Fondo de Investigaciones Sanitarias (PI 04 0067), and in part by Junta de Andalucía, grupo CTS-180. G. Orozco, PhD; E. Sánchez, PhD, Instituto de Parasitología y Biomedicina; M.A. González-Gay, MD, Hospital Xeral-Calde, M.A. López-Nevot, MD, Servicio de Inmunología, Hospital Virgen de las Nieves; B. Torres, PhD, Servicio de Inmunología, Hospital Virgen del Rocío; D. Pascual-Salcedo, MD; A. Balsa, MD, Servicio de Reumatologia e Inmunología, Hospital La Paz; J.L. Pablos, MD; A. García, MD, Servicio de Reumatología, Hospital Virgen de las Nieves; F. González-Escribano, PhD, Servicio de Inmunología, Hospital Virgen del Rocío; J. Martín, MD, PhD, Instituto de Parasitología y Biomedicina. Dr. Orozco and Dr. Sánchez contributed equally to this study. Address reprint requests to Dr. J. Martín, Instituto de Parasitología y Biomedicina, CSIC, Parque Tecnológico de Ciencias de la Salud, Avenida del Conocimiento s/n 18100-Armilla, Granada, Spain. E-mail: martin@ipb.csic.es Accepted for publication February 27, 2006.
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