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HLA-DRB1*04 Alleles in Japanese Rheumatoid Arthritis Patients with AA Amyloidosis
KIYOSHI MIGITA, TADASHI NAKAMURA, YUMI MAEDA, TAICHIRO MIYASHITA, TOMOKI ORIGUCHI, HIROSHI YATSUHASHI, MINORU NAKAMURA, HIROMI ISHIBASHI, and KATSUMI EGUCHI ABSTRACT. Objective. To compare the HLA-DRB1 shared epitope (SE) alleles in Japanese patients with rheumatoid arthritis (RA) and amyloid A (AA) amyloidosis versus those without AA amyloidosis. Methods. The HLA-DRB1 alleles were genotyped for 91 RA patients without AA amyloidosis, 33 RA patients with AA amyloidosis, and 63 control subjects. HLA-DRB1 typing was performed by polymerase chain reaction, sequence-specific oligonucleotide probe hybridization method. Results. Although a significant difference was not observed, the frequency of SE genotype was higher in RA patients with AA amyloidosis than in those without AA amyloidosis. All SE-positive RA patients with AA amyloidosis had *04 alleles (*0401, *0405, *0410), and a significant association of the presence of a double dose of *04 SE alleles with AA amyloidosis (OR 4.0, 95% CI 1.91–13.99) was observed. Conclusion. Our data suggest that presence of double *04 SE is associated with a higher risk of developing AA amyloidosis in Japanese patients with RA. (J Rheumatol 2006;33:2120-3) Key Indexing Terms:
AA AMYLOIDOSIS From the Clinical Research Center and Department of Rheumatology, NHO Nagasaki Medical Center, Omura; the Kumamoto Center for Arthritis and Rheumatology, Kumamoto; and First Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan. Supported by Health and Labour Sciences Research Grants from the Ministry of Health, Labour and Welfare of Japan. K. Migita, MD, Clinical Research Center and Department of Rheumatology, NHO Nagasaki Medical Center; T. Nakamura, MD, Kumamoto Center for Arthritis and Rheumatology; Y. Maeda, MSc; T. Miyashita, MD, Clinical Research Center and Department of Rheumatology, NHO Nagasaki Medical Center; T. Origuchi, MD, First Department of Internal Medicine, Nagasaki University School of Medicine; H. Yatsuhashi, MD; M. Nakamura, MD; H. Ishibashi, MD, Clinical Research Center and Department of Rheumatology, NHO Nagasaki Medical Center; K. Eguchi, MD, First Department of Internal Medicine, Nagasaki University School of Medicine. Address reprint requests to Dr. K. Migita, Clinical Research Center, NHO Nagasaki Medical Center, Kubara 2-1001-1, Omura 856-8652, Japan. E-mail: migita@nmc.hosp.go.jp Accepted for publication June 1, 2006.
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