 |
|
|
 |
Urokinase-type Plasminogen Activator, Receptor, and Inhibitor
Correlating with Gelatinase-B (MMP-9) Contribute to Inflammation in Gouty
Arthritis of the Knee
SHU-CHEN CHU, SHUN-FA YANG, KO-HUANG LUE, YIH-SHOU HSIEH, TANG-YAN HSIAO, and KO-HSIU LU
ABSTRACT. Methods. We compared the antigenic values of urokinase-type PA (uPA), soluble uPA receptor (suPAR), and PA inhibitor type-1 (PAI-1) and the levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 in 63 synovial fluid (SF) samples from knee joints of 38 patients with gouty arthritis and 20 SF samples from knee joints of 20 patients with osteoarthritis (OA). Results. The increases of uPA, suPAR, and PAI-1 antigenic values in SF were associated with increased levels of latent MMP-9 (pro-MMP-9) (p < 0.001, p < 0.001, p < 0.001, respectively) in gouty arthritis, whereas a correlation was not observed between uPA, suPAR, and PAI-1 antigenic values and MMP-2 levels. Increased uPA, suPAR, and PAI-1 antigenic values in gouty arthritis SF were also correlated to each other (p < 0.001, p < 0.001, p < 0.001). In gouty arthritis SF, no significant relations were observed between uPA and suPAR antigenic values and leukocyte, neutrophil, or monocyte counts, while increased values of PAI-1 corresponded closely with increased leukocyte and neutrophil counts (p = 0.005, p = 0.004). Significantly higher values of the uPA, suPAR, and PAI-1 (p < 0.001, p < 0.001, p = 0.012) appeared in SF of gouty arthritis than in SF from patients with OA. Conclusion. These results show a correlation of the PA/plasmin system and MMP-9 in SF from patients with gouty arthritis, resulting in increased PA catalytic activity and contributing to inflammation in gouty arthritis. (J Rheumatol 2006;33:311-7) Key Indexing Terms:
PLASMINOGEN ACTIVATOR
From the Institute of Biochemistry, School of Medicine, Chung Shan Medical University; Department of Orthopaedic Surgery, Chung Shan Medical University Hospital; and Department of Food Science, Chungtai Institute of Health Sciences and Technology, Taichung, Taiwan. Supported by grant from the Research Section of Chung Shan Medical University (CSMU 93-OM-B-029) and National Science Council, Taiwan (NSC94-2314-B-040-009). S-C. Chu, PhD, Department of Food Science, Chungtai Institute of Health Sciences and Technology; S-F. Yang, PhD, Institute of Biochemistry; K-H. Lue, MD, School of Medicine; Y-S. Hsieh, PhD, Institute of Biochemistry, Chung Shan Medical University; T-Y. Hsiao, MD; K-H. Lu, MD, PhD, Department of Orthopaedic Surgery, Chung Shan Medical University Hospital; School of Medicine, Chung Shan Medical University. Address reprint requests to Dr. K-H. Lu, 110, Section 1, Chien-Kuo N. Road, Taichung 402, Taiwan. E-mail: cshy307@csh.org.tw Accepted for publication August 29, 2005.
|
