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Toll-like Receptor Expression in Lupus Peripheral Blood Mononuclear Cells
KIYOSHI MIGITA, TAICHIRO MIYASHITA, YUMI MAEDA, MINORU NAKAMURA, HIROSHI YATSUHASHI, HIRONORI KIMURA, HIROMI ISHIBASHI, and KATSUMI EGUCHI ABSTRACT. Objective. To investigate expression of members of the Toll-like receptor (TLR) family in peripheral blood mononuclear cells (PBMC) in patients with systemic lupus erythematosus (SLE). Methods. We analyzed PBMC from 14 patients with SLE and 15 healthy subjects. The surface expressions of TLR2 and TLR4 and intracellular expression of TLR9 on PBMC were analyzed by flow cytometry. Results. Although TLR4 expressions on CD14+ monocytes were not significantly different between healthy subjects and patients with SLE, TLR2 expressions on monocytes were reduced in patients with SLE compared to healthy subjects. Intracellular TLR9 expression levels of CD19+ B lymphocytes were significantly elevated in patients with SLE. However, the TLR9 expression levels of plasmacytoid dendritic cells were not significantly different between these patients and healthy subjects. Conclusion. Our results show that human peripheral blood B cells express TLR9 and that its expression is increased in patients with SLE. This upregulated expression of TLR9 in B cells may be related to the abnormal B cell hyperactivity in patients with SLE. (First Release Jan 15 2007; J Rheumatol 2007;34:493–500) Key Indexing Terms:
B LYMPHOCYTES From the Clinical Research Center and Department of General Internal Medicine, NHO Nagasaki Medical Center, Omura; and First Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan. K. Migita, MD, Clinical Research Center and Department of General Internal Medicine; Y. Maeda, MSc; M. Nakamura, MD; H. Yatsuhashi, MD; H. Ishibashi, MD, Clinical Research Center; T. Miyashita, MD; H. Kimura, MD, Department of General Internal Medicine, NHO Nagasaki Medical Center; K. Eguchi, MD, First Department of Internal Medicine, Nagasaki University School of Medicine. Address reprint requests to Dr. K. Migita, Clinical Research Center, NHO Nagasaki Medical Center, Kubara 2-1001-1, Omura 856-8652, Japan. E-mail: migita@nmc.hosp.go.jp Accepted for publication October 25, 2006.
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