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Abnormal Transforming Growth Factor-ß Expression in Mesenchymal Stem Cells from Patients with Osteoarthritis RAQUEL ROLLÍN, ROBERTO ÁLVAREZ-LAFUENTE, FERNANDO MARCO, JOSE ANTONIO GARCÍA-ASENJO, JUAN ÁNGEL JOVER, LUIS RODRÍGUEZ, LUIS LÓPEZ-DURÁN, and BENJAMÍN FERNÁNDEZ-GUTIÉRREZ
ABSTRACT. Methods. Total RNA was extracted from primary cultures of MSC and quantitative real-time reverse transcription-polymerase chain reaction was performed to analyze gene expression. Results. MSC from patients with OA showed significantly increased total TGF-ß, TGF-ß1 isoform, TBR-II, and TBR-III mRNA expression compared to controls. Conclusion. Our study is the first reporting the gene expression levels of TGF-ß and its isoforms and receptors in patients with OA. These findings might have pathological significance for OA disease. (First Release April 1 2008; J Rheumatol 2008;35:904–6) Key Indexing Terms:
OSTEOARTHRITIS
From Servicio de Cirugía Ortopédica y Traumatología, Servicio de Neurología, Servicio de Anatomía Patológica, and Servicio de Reumatología, Hospital Clínico San Carlos, Madrid, Spain. Supported by grants from the FMMA and FIS 04/1698. Dr. Rollin holds a research contract of the Fundación para la Investigación Biomédica-Hospital Clínico San Carlos Madrid. R. Rollín, PhD, Cirugía Ortopédica y Traumatología; R. Álvarez-Lafuente, PhD, Neurología; F. Marco, MD, PhD, Cirugía Ortopédica y Traumatología; J.A. García-Asenjo, MD, PhD, Anatomía Patológica; J.Á. Jover, MD, PhD; L. Rodríguez, MD; Reumatología; L. López-Durán, MD, PhD, Cirugía Ortopédica y Traumatología; B. Fernández-Gutiérrez, MD, PhD, Reumatología, Hospital Clínico San Carlos. Address reprint requests to Dr. B. Fernández-Gutiérrez, Servicio de Reumatología, Hospital Clínico San Carlos, C/ Profesor Martín Lagos s/n, 28040 Madrid, Spain. E-mail: bfernandez.hcsc@salud.madrid.org Accepted for publication December 11, 2007.
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